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KMID : 0879419990010010033
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Journal of the Implant Research
1999 Volume.1 No. 1 p.33 ~ p.42
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TEM Study of Bone-Implant interface
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Abstract
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One of the main research in dental implant science is the histological explanation of the establishment of an interface between tissue and implant materiols.
The primary function of a bone-implant interface is to provide safe and effective load transfer from dental implant to Jaw bone, and loosening of this interfacial fixation is the leading cause of dental implant failure.
The purpose of this study was to get clinical and histopathological informations of failed 1MZ implant removed from the 60 years old male patient at 3 years after implantation. Implant and peri-implant tissue on upper right central incisor area were removed because of radiolucency and mobility. Immediately fixed in phosphate-buffered(PH7.4) 2.5% glutaraldehyde. The specimens were prepared with H-E stain for LM. And for TEM, after post fixation specimen were fixed in 1% osmium tetroxide for one hour, followed by dehydration with increasing concentrations of ethyl alcohol and prophylene oxide. Embedding was in Epon 812 and semi-thin section was stained with toluidine blue, thin section with uranyl acetate and lead citrate, and observed, photographed with TEM.
The obtained results were as follows:
1. Epitherial downgrowth to the apex of implant was observed.
2. Generally, epithelial width of upper part of implant was thicker than lower and epithelial tissue has no digitation form.
3. The loose fibrous connective tissue with inflammatory cells was observed beneath the epithelium.
4. Near the epithelial invagition, in connective tissue showed inflammatory cells with mast cell.
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